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tryple™ express enzyme cell dissociation reagent  (Thermo Fisher)


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    Thermo Fisher tryple™ express enzyme cell dissociation reagent
    Tryple™ Express Enzyme Cell Dissociation Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+dissociation+reagent+tryple+express+enzyme/bio_rxiv__2025__01__22__634029-154-12-18
    Average 90 stars, based on 1 article reviews
    tryple™ express enzyme cell dissociation reagent - by Bioz Stars, 2026-09
    90/100 stars

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    Isolation:

    Article Title: Myoepithelial cells are a dynamic barrier to epithelial dissemination.
    Article Snippet: .. After isolation of K8::Cre-ER;R26::LSL-rtTA;TRE-Twist1(mT/ mG) organoids, 0.5–1 ml of a 10-ml organoid suspension was incubated with a cell dissociation reagent TrypLE Express Enzyme (1×; 12604013; Thermo Fisher Scientific) for 5–10 min to dissociate organoids into single cells. .. Trypan Blue Solution (152250061; Thermo Fisher Scientific) was added to the dissociated cell solution, and a hematocytometer was used to count the number of cells.

    Article Title: Myoepithelial cells are a dynamic barrier to epithelial dissemination
    Article Snippet: .. After isolation of K8::Cre-ER;R26::LSL-rtTA;TRE-Twist1(mT/mG) organoids, 0.5–1 ml of a 10-ml organoid suspension was incubated with a cell dissociation reagent TrypLE Express Enzyme (1×; 12604013; Thermo Fisher Scientific) for 5–10 min to dissociate organoids into single cells. .. Trypan Blue Solution (152250061; Thermo Fisher Scientific) was added to the dissociated cell solution, and a hematocytometer was used to count the number of cells.

    Suspension:

    Article Title: Myoepithelial cells are a dynamic barrier to epithelial dissemination.
    Article Snippet: .. After isolation of K8::Cre-ER;R26::LSL-rtTA;TRE-Twist1(mT/ mG) organoids, 0.5–1 ml of a 10-ml organoid suspension was incubated with a cell dissociation reagent TrypLE Express Enzyme (1×; 12604013; Thermo Fisher Scientific) for 5–10 min to dissociate organoids into single cells. .. Trypan Blue Solution (152250061; Thermo Fisher Scientific) was added to the dissociated cell solution, and a hematocytometer was used to count the number of cells.

    Article Title: Myoepithelial cells are a dynamic barrier to epithelial dissemination
    Article Snippet: .. After isolation of K8::Cre-ER;R26::LSL-rtTA;TRE-Twist1(mT/mG) organoids, 0.5–1 ml of a 10-ml organoid suspension was incubated with a cell dissociation reagent TrypLE Express Enzyme (1×; 12604013; Thermo Fisher Scientific) for 5–10 min to dissociate organoids into single cells. .. Trypan Blue Solution (152250061; Thermo Fisher Scientific) was added to the dissociated cell solution, and a hematocytometer was used to count the number of cells.

    Incubation:

    Article Title: Myoepithelial cells are a dynamic barrier to epithelial dissemination.
    Article Snippet: .. After isolation of K8::Cre-ER;R26::LSL-rtTA;TRE-Twist1(mT/ mG) organoids, 0.5–1 ml of a 10-ml organoid suspension was incubated with a cell dissociation reagent TrypLE Express Enzyme (1×; 12604013; Thermo Fisher Scientific) for 5–10 min to dissociate organoids into single cells. .. Trypan Blue Solution (152250061; Thermo Fisher Scientific) was added to the dissociated cell solution, and a hematocytometer was used to count the number of cells.

    Article Title: Myoepithelial cells are a dynamic barrier to epithelial dissemination
    Article Snippet: .. After isolation of K8::Cre-ER;R26::LSL-rtTA;TRE-Twist1(mT/mG) organoids, 0.5–1 ml of a 10-ml organoid suspension was incubated with a cell dissociation reagent TrypLE Express Enzyme (1×; 12604013; Thermo Fisher Scientific) for 5–10 min to dissociate organoids into single cells. .. Trypan Blue Solution (152250061; Thermo Fisher Scientific) was added to the dissociated cell solution, and a hematocytometer was used to count the number of cells.



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